The aim of this project is to investigate whether the survival difference between tumours with canonical and non-canonical IDH mutations can be explained by higher D-2HG concentrations. To this end, we will:
- Demonstrate that increased D-2HG concentrations are directly correlated to the survival advantage of astrocytoma patients.
2. Demonstrate that pharmacological and genetic interventions that alter intracellular D-2HG concentrations directly affect cell-viability in IDH-mutated astrocytoma cultures.