Know who you are listening to – Identifying neurons in real-time during electrophysiology

Project summary

Our brain contains hundreds of different neurons that communicate with each other through electrochemical signals that can be analyzed with patch clamp electrophysiology (PCE). PCE has enabled us to understand how neurons communicate, learn, and remember. Because it is unknown which type of neuron is analyzed during a PCE experiment, PCE requires inefficient, laborious steps to identify the neuron afterwards. We will develop a technology called RISEE (Rapid Identification of neuronal Subtypes during patch clamp Electrophysiology by CRISPR Endonucleases) to identify neurons through fluorescent signals during PCE.

RISEE will simplify and accelerate PCE and become an indispensable tool for neuroscientists.